Taxadiene synthase
| taxadiene synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 4.2.3.17 | ||||||||
| CAS no. | 169277-52-5 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
The enzyme taxadiene synthase (EC 4.2.3.17) catalyzes the chemical reaction
- geranylgeranyl diphosphate taxa-4,11-diene + diphosphate
This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on phosphates. The systematic name of this enzyme class is geranylgeranyl-diphosphate diphosphate-lyase (cyclizing, taxa-4,11-diene-forming). Other names in common use include geranylgeranyl-diphosphate diphosphate-lyase (cyclizing, and taxadiene-forming). This enzyme participates in diterpenoid biosynthesis. The crystal structure of the enyme was the first of a diterpene cyclase and was reported by the research group of David W. Christianson at the University of Pennsylvania.[1]
References
- ^ Köksal, Mustafa; Jin, Yinghua; Coates, Robert M.; Croteau, Rodney; Christianson, David W. (2011). "Taxadiene synthase structure and evolution of modular architecture in terpene biosynthesis". Nature. 469 (7328): 116–120. Bibcode:2011Natur.469..116K. doi:10.1038/nature09628. PMC 3059769. PMID 21160477.
- Croteau R; Hezari, M; Zajicek, J; Vogel, BS; Lafever, RE; Lewis, NG; Croteau, R (1995). "Cyclization of geranylgeranyl diphosphate to taxa-4(5),11(12)-diene is the committed step of taxol biosynthesis in Pacific yew". J. Biol. Chem. 270 (15): 8686–90. Bibcode:1995JBiCh.270.8686K. doi:10.1074/jbc.270.15.8686. PMID 7721772.
- Hezari M, Lewis NG, Croteau R (1995). "Purification and characterization of taxa-4(5),11(12)-diene synthase from Pacific yew (Taxus brevifolia) that catalyzes the first committed step of taxol biosynthesis". Arch. Biochem. Biophys. 322 (2): 437–44. doi:10.1006/abbi.1995.1486. PMID 7574719.
- Lin X, Hezari M, Koepp AE, Floss HG, Croteau R (1996). "Mechanism of taxadiene synthase, a diterpene cyclase that catalyzes the first step of taxol biosynthesis in Pacific yew". Biochemistry. 35 (9): 2968–77. doi:10.1021/bi9526239. PMID 8608134.
- Hezari M, Ketchum RE, Gibson DM, Croteau R (1997). "Taxol production and taxadiene synthase activity in Taxus canadensis cell suspension cultures". Arch. Biochem. Biophys. 337 (2): 185–90. doi:10.1006/abbi.1996.9772. PMID 9016812.
- Coates RM, Williams RM, Croteau R (2000). "Intramolecular proton transfer in the cyclization of geranylgeranyl diphosphate to the taxadiene precursor of taxol catalyzed by recombinant taxadiene synthase". Chem. Biol. 7 (12): 969–77. doi:10.1016/S1074-5521(00)00046-6. PMID 11137819.