Fusicocca-2,10(14)-diene synthase
| Fusicocca-2,10(14)-diene synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 4.2.3.43 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Fusicocca-2,10(14)-diene synthase (EC 4.2.3.43, fusicoccadiene synthase, PaFS, PaDC4) is an enzyme with systematic name geranylgeranyl diphosphate-lyase (fusicocca-2,10(14)-diene-forming).[1] This enzyme catalyses the following chemical reaction
- geranylgeranyl diphosphate fusicocca-2,10(14)-diene + diphosphate
This multifunctional enzyme also has EC 2.5.1.29, farnesyltranstransferase, activity. In 2016, the crystal structures of individual prenyltransferase and cyclase domains were reported by the research group of David W. Christianson at the University of Pennsylvania.[2] The structure of the intact, full-length enzyme was studied using negative-stain electron microscopy and cryo-electron microscopy, showing that a central prenyltransferase octamer was surrounded by eight randomly splayed-out cyclase domains capable of transient association with the prenyltransferase.[3][4]
References
- ^ Toyomasu T, Tsukahara M, Kaneko A, Niida R, Mitsuhashi W, Dairi T, Kato N, Sassa T (February 2007). "Fusicoccins are biosynthesized by an unusual chimera diterpene synthase in fungi". Proceedings of the National Academy of Sciences of the United States of America. 104 (9): 3084–8. Bibcode:2007PNAS..104.3084T. doi:10.1073/pnas.0608426104. PMC 1805559. PMID 17360612.
- ^ Chen, Mengbin; Chou, Wayne K. W.; Toyomasu, Tomonobu; Cane, David E.; Christianson, David W. (2016). "Structure and Function of Fusicoccadiene Synthase, a Hexameric Bifunctional Diterpene Synthase". ACS Chemical Biology. 11 (4): 889–899. doi:10.1021/acschembio.5b00960. PMC 4833508. PMID 26734760.
- ^ Faylo, Jacque L.; Van Eeuwen, Trevor; Kim, Hee Jong; Gorbea Colón, Jose J.; Garcia, Benjamin A.; Murakami, Kenji; Christianson, David W. (2021). "Structural insight on assembly-line catalysis in terpene biosynthesis". Nature Communications. 12 3487. Bibcode:2021NatCo..12.3487F. doi:10.1038/s41467-021-23589-9. PMC 8190136. PMID 34108468.
- ^ Faylo, Jacque L.; Van Eeuwen, Trevor; Gupta, Kushol; Murakami, Kenji; Christianson, David W. (2022). "Transient Prenyltransferase–Cyclase Association in Fusicoccadiene Synthase, an Assembly-Line Terpene Synthase". Biochemistry. 61 (21): 2417–2430. doi:10.1021/acs.biochem.2c00509. PMC 9648990. PMID 36227241.
External links
- Fusicocca-2,10(14)-diene+synthase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)